Diare dapat disebabkan oleh berbagai penyebab langsung maupun faktor risiko, salah satunya infeksi bakteri E. coli. Penelitian bertujuan mengetahui hubungan E. colipada peralatan makan balita dengan diare pada balita. Penelitian juga melihat faktorterkait dengan kontaminasi E. coli, meliputi sarana air bersih, serta pengetahuan dan praktik higiene sanitasi pengasuh balita. Studi kasus kontrol dilakukan di wilayahkerja Puskesmas Tugu pada minggu terakhir bulan April sampai minggu kedua bulanMei 2014. Sebanyak 30 sampel kasus didapatkan dari kunjungan pasien balita penderita diare ke Puskesmas Tugu dan 30 sampel kontrol merupakan balita yang tinggal dekat dengan sampel kasus (tetangga). Pengumpulan data meliputi wawancara, observasi, dan sampel usap alat. Pemeriksaan E. coli menggunakan Sanitakun dan dianalisis di laboratorium. Penelitian menunjukkan keberadaan E. colipada peralatan makan balita tidak berhubungan signifikan dengan diare (p value =0,108; OR = 7,250; CI = 0,815-64,457). Terdapat dua variabel yang berhubungan signifikan dengan diare, yaitu jarak sumber air ke sumber pencemar (p value = 0,009;OR = 4,929; CI = 1,612-15,071) dan pengetahuan pengasuh balita (p value = 0,019;OR = 4,125; CI = 1,387-12,270). Jenis sarana air bersih, praktik pencucian peralatan makan balita, dan praktik CTPS pengasuh balita tidak berhubungan signifikan dengan diare pada balita di Kelurahan Tugu.
Kata kunci:Diare, balita, Escherichia coli, air bersih, peralatan makan balita

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Diarrhea can caused by direct causes as well as risk factors, one of them isE. coli bacteria infection. This study aims to know the correlation betweenE. coli on children under five‟s eating utensils with diarrhea in childrenunder five. This study also observed another risk factors related to E. colicontamination, including good water supply, and caregiver‟s knowledge andhygiene sanitation practices. A case control study was conducted incoverage area of Tugu Community Health Center on the last week of Apriluntil the second week of May, 2014. As many as 30 case samples obtainedfrom children under five‟s diarrhea patient‟s visit to Tugu CommunityHealth Center and 30 control samples are children under five who live nextto case samples (neighbour). Data collecting including interview,observation, and swab sample. E. coli was checked using Sanitakun andanalyzed in laboratory. Study shows that existence of E. coli on childrenunder five‟s eating utensils is not significantly associated with diarrhea (pvalue = 0,108; OR = 7,250; CI = 0,815-64,457). There are two variableswhich significantly associated with diarrhea, they are the distance of goodwater source from contaminant source (p value = 0,009; OR = 4,929; CI =1,612-15,071) and caregiver‟s knowlegde (p value = 0,019; OR = 4,125; CI= 1,387-12,270). Good water supply, washing eating utensils practice, andcaregiver‟s washing hands using soap practice are not associated withdiarrhea in children under five in Tugu Village.
Key words:Diarrhea, children under five, Escherichia coli, good water, children underfive‟s eating utensils