Strategi penanggulangan TB melalui strategi DOTS (Directly Observed Trearmem Shorlcourse) memprioritaskan penemuan pasien melalui pemeriksaan mikroskopis, oleh karena itu mutu pemeriksaan mikroskopis perlu dipantau tems. Hasil pemeriksaan mikroskopis sputum BTA ,oleh 54 pemugas laboratorium puskesmas (Puskesmas Ruiukan Mikroskopis dan Puskesmas Pelaksana Mandiri) di Provinsi Jambi pada tahun 2004 ada 29 puskesmas yang hasil error rate 25%, sedangkan pada tahun 2005 mcnjndi 32 puskcsmas yang hasil error rare-nya 25%. Untuk itu pcrlu dilakukan penilaian terhadap faktor-faktor yang berhubungan dengan mutu pemeiiksaan mikroskopis sputum BTA. Penelitian ini bertuiuan untuk mengetahui gambaran dan faktor-faktor yang berhubungan dengan mutu pemeriksaan mikroskopis sputum BTA pada laboratorium puskesmas (PRM dan PPM) di Provinsi Jambi tahun 2006, dengan menggunakan metodologi kuantitatif yang bersifat deskriptif dengam desaiu” penelitian berupa pcndckatan cross sectional, terhadap 56 petugas laboratorium puskesmas di PRM dan PPM (total populasi). Hasil pemeriksaan mikroskopis sputum BTA yang bermutu baik masih rendah, hanya 35,7%. Adapun faktor yang berhubungan signiiikan dengan mutu pemeriksaan mikroskopis sputum BTA adalah pelatihan (tanpa dikontrol), dan faktor pengalaman kelfia, supervisi, kepuasan kerja, dan penerapan SOP (dengan dikontrol). Faktor yang paling dominan bcrhubungan dengan rnutu pemeriksaan mikroskopis sputum BTA aclalah pencmpan SOP. Disarankan kepada puskcsmas agar petugas laboratorium selalu menerapkan SOP, meqiaga keamanan bckclja di laboratorium, dan merawat mikroskop dcngan bai[c. Kepada Dinas Kesehatan K.abupatenfKota agar pembinaan petugas laboratodum dilakukan torus-mcncms melalui peiaksanaan supenkisi yang baik. Kepada Dinas Kesehatan Provinsi agar dapat rpelatih semua petugas laboratoriurn puskesmas, melaksanakan pertemuan untuk pembinaan dan pcrnbekalan pengetahuan terhadap petugas TB kabupatenfkota dan petugas laboratorium puskesmas, dan juga perlu bckcrjasama dengan Balai Laboratorium Kesehatan untuk melakukan pembinaan di puskesmas (PRM dan PPND.

TB prevention strategy with DOTS (Directly Observed Treatment Short course) give priority to patient’s invention by microscopic examination, therefore we must always control the microscopic examination. The result of BTA sputum microscopic examination by 54 government clinic laboratory assistant (Microscopic Reconciliation Government Clinic/PRM and Autonomy Execution Government Clinic/PPM) in Province of Jamb in year 2004, there was 29 local govemment clinic with error rate 25%, whereas in 2005 became 32 local government clinic with error rate 25%. Because of that, we need to evaluate about factors which related with quality of BTA sputum microscopic examination. The purpose of the research is to get the description and factors that related with quality of BTA sputum microscopic control, at PRM and PPM laboratories in Province of Jambi, in year 2006, by using quantitative methodology, which have descriptive characteristic with cross sectional approaching research design, toward 56 laboratory assistant at PRM and PPM (total population). The result of BTA sputum microscopic examination with good quality is still low, that is only 35.7% The factors that have a significant relation with quality of BTA sputum microscopic examination are training (without controlling), and work experience factor, supervision, work satisfaction, and SOP implementation (without controlling). The most dominant factor which related with quality of BTA sputum microscopic examination is SOP examination. We suggest to government clinic is laboratory assistant must implement SOP, maintain the security of laboratory, take good care of microscope. For public service in Regency, they must train laboratory assistant continually with good supervision. For public service in Province, they must train all laboratory assistant of local government clinic by meeting for founded and provided knowledge towards TB Regency officer and laboratory assistant of public government clinic, and also good cooperate with Health Laboratory Center to make founding at local government clinic (PRM and PPM).